nuclease protection assay การใช้

• Nuclease protection assays are used to map introns and transcription of the gene in the cell.
• To address these problems, Rhee and Pugh revised the classic nuclease protection assay to develop ChIP-exo.
• Primer extension offers an alternative to a nuclease protection assay ( S1 nuclease mapping ) for quantifying and mapping RNA transcripts.
• Because RNase H specifically degrades only the RNA in RNA : DNA hybrids, it is commonly used in molecular biology to destroy the RNA template after first-strand complementary DNA ( cDNA ) synthesis by reverse transcription, as well as in procedures such as nuclease protection assays.
• It is slower and less quantitative, but also produces accurate information about the " size " of the target RNA . Nuclease protection assay products are limited to the size of the initial probes due to the destruction of the non-hybridized RNA during the nuclease digestion step.